Unlabeled antibody peroxidase-antiperoxidase method combined with direct immunofluorescence.
نویسندگان
چکیده
منابع مشابه
Immunoreactive prolactin in epithelial cells of normal and cancerous human breast and prostate detected by the unlabeled antibody peroxidase-antiperoxidase method.
A rabbit antiserum to ovine prolactin was used with the peroxidase-antiperoxidase method to search for immunoreactive prolactin in normal and pathological human breast and prostate. We first substantiated that the heterologous antiserum could recognize human prolactin. When sections of human anterior pituitary were exposed to the anti-prolactin serum, groups of angular-shaped cells were selecti...
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The intralobular distribution of nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c (P-450) reductase (NADPH:ferricytochrome oxidoreductase, EC 1.6.2.4) in rat liver has been investigated by means of two quantitative immunohistochemical techniques: microdensitometric quantitation of unlabeled antibody peroxidaseantiperoxidase staining and microfluorometric analysis of indirect flu...
متن کاملEffect of formalin pigment removal on peroxidase-antiperoxidase immunoperoxidase technique.
In immunoperoxidase preparations formalin pigment (acid formaldehyde haematein) may be difficult to distinguish from the brown staining of the 3,3'-diaminobenzidine (DAB) reaction product. The pigment, produced when formalin is in contact with blood rich tissues, takes the form of dark brown microcrystals that have the ability to rotate the plane of polarised light.' Even though formalin pigmen...
متن کاملComparison of methods for immunocytochemical detection of rotavirus infections.
Rotavirus infections in intestinal tissues of animals or in tissue culture cells were detected by the immunocytochemical unlabeled soluble enzyme peroxidase antiperoxidase method. Comparison of the immunofluorescence and peroxidase antiperoxidase immunological staining techniques revealed that the two methods are equally sensitive for detection of rotavirus-infected cells. The peroxidase antipe...
متن کاملDirect and indirect immunofluorescence.
Immunofluorescence is a valuable auxiliary diagnostic tool for autoimmune bullous diseases and inflammatory disorders, since their clinical and histopathologic findings may be inconclusive. It is a feasible laboratory method that requires experienced technicians and detects in situ and circulating immune deposits that may be involved in the pathogenesis of such skin diseases.
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1981
ISSN: 0022-1554,1551-5044
DOI: 10.1177/29.6.6114118